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Fig. 4. The mitochondrial activity of human umbilical vein endothelial cells (HUVECs) was quantified using a WST cell viability assay. HUVECs were cultured on alginate-based hydrogels with and without encapsulated VEGF and normalized using non-modified cell controls: methacrylated alginate (AM), methacrylated alginate-RGD (AMR), methacrylated alginate-heparin (AMH), methacrylated alginate-RGD-heparin (AMRH). An absorbance reading was taken after 24 h of culture; (n = 4; mean ± standard deviation; *p < 0.05, **p < 0.005).

Journal: Journal of the mechanical behavior of biomedical materials

Article Title: Bio-inspired green light crosslinked alginate-heparin hydrogels support HUVEC tube formation.

doi: 10.1016/j.jmbbm.2021.104932

Figure Lengend Snippet: Fig. 4. The mitochondrial activity of human umbilical vein endothelial cells (HUVECs) was quantified using a WST cell viability assay. HUVECs were cultured on alginate-based hydrogels with and without encapsulated VEGF and normalized using non-modified cell controls: methacrylated alginate (AM), methacrylated alginate-RGD (AMR), methacrylated alginate-heparin (AMH), methacrylated alginate-RGD-heparin (AMRH). An absorbance reading was taken after 24 h of culture; (n = 4; mean ± standard deviation; *p < 0.05, **p < 0.005).

Article Snippet: HUVECs and vascular endothelial cell culture medium were purchased from ATCC (ATCC® CRL-1730TM, ATCC® PCS-100-030TM).

Techniques: Activity Assay, Viability Assay, Cell Culture, Modification, Standard Deviation

Fig. 5. Total protein content of the population of human umbilical vein endothelial cells (HUVECs) cultured on various hydrogel groups was quantified using a Pierce protein assay. Cells were cultured on alginate-based hydrogels with and without encapsulated VEGF and compared to non-modified cell controls: meth acrylated alginate (AM), methacrylated alginate-RGD (AMR), methacrylated alginate-heparin (AMH), methacrylated alginate-RGD-heparin (AMRH). The length of culture time and the material group played a significant role in total protein content (A–D). There were no significant differences between -VEGF and +VEGF hydrogels regarding effect on total protein concentration: (E) AM; (F) AMH; (G) AMR; (H) AMRH. Protein absorbance and quantification was performed at 6, 12, 24, and 72 h; (n = 4; mean ± standard deviation; *p < 0.05, **p < 0.005).

Journal: Journal of the mechanical behavior of biomedical materials

Article Title: Bio-inspired green light crosslinked alginate-heparin hydrogels support HUVEC tube formation.

doi: 10.1016/j.jmbbm.2021.104932

Figure Lengend Snippet: Fig. 5. Total protein content of the population of human umbilical vein endothelial cells (HUVECs) cultured on various hydrogel groups was quantified using a Pierce protein assay. Cells were cultured on alginate-based hydrogels with and without encapsulated VEGF and compared to non-modified cell controls: meth acrylated alginate (AM), methacrylated alginate-RGD (AMR), methacrylated alginate-heparin (AMH), methacrylated alginate-RGD-heparin (AMRH). The length of culture time and the material group played a significant role in total protein content (A–D). There were no significant differences between -VEGF and +VEGF hydrogels regarding effect on total protein concentration: (E) AM; (F) AMH; (G) AMR; (H) AMRH. Protein absorbance and quantification was performed at 6, 12, 24, and 72 h; (n = 4; mean ± standard deviation; *p < 0.05, **p < 0.005).

Article Snippet: HUVECs and vascular endothelial cell culture medium were purchased from ATCC (ATCC® CRL-1730TM, ATCC® PCS-100-030TM).

Techniques: Cell Culture, Modification, Protein Concentration, Standard Deviation